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. 2023 Aug 7;24(15):12532. doi: 10.3390/ijms241512532

Figure 4.

Figure 4

Expression pattern of LerHMGRs in different tissues and cultured L. erythrorhizon callus cells/hairy roots. (A) Heat map showing the expression profile of LerHMGRs in different tissues of L. erythrorhizon using transcriptome data. Dark: M9 and darkness-cultured hairy roots; Light: B5 and light-cultured hairy roots; MR: mature root; PD: periderm of mature root; FL: flowers; ML: leaves + stems; CT: cortex of mature root; SE: stele of mature root. The number in the grid is the TPM value of each gene in different tissues and under different treatments. The expression values were normalized by Log2 (TPM + 1) to create the heat map, using the average TPM of three biological replicates. (B) The expression level of LerHMGRs in the leaf, stem, and root of L. erythrorhizon was analyzed using qPCR. (C) The expression level of LerHMGRs in L. erythrorhizon cells cultured in M9 and darkness for different times (0 h, 6 h, 1 day, 3 day, and 6 day) was detected using qPCR. The error bars indicate the SDs of three replicates. Asterisks represent significant differences via the Student’s t-test analysis (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001) as compared with root in (B) and 0 h in (C), respectively.