Fig 2. Ploidy detection by flow cytometry and chromosomal counting.

Flow cytometry histograms of nuclei extracted from young leaf tissue of mulberry accessions comprising different ploidy groups and the corresponding chromosome numbers such as (A) diploid (2n = 2x = 28) (B) triploid (2n = 3x = 42), (C) tetraploid (2n = 4x = 56) and (D) hexaploid (2n = 6x = 84) with flow cytometry histograms depicting the relative fluorescence intensity obtained from the simultaneous analysis of isolated nuclei of mulberry leaves and the reference standard (Pisum sativum). Distinguishable predicted ploidy levels presented as histogram (E) and scattered plot (F). Average for the standards = 3.9% and for the sample = 2.9%.