FIG. 1.

Demonstration of nucleic acid chaperone activity by selective annealing of oligonucleotides. (A) Oligonucleotides were incubated alone (lanes 1 and 2) or with 0.01, 0.04, or 0.16 μg of NC (lanes 3 and 4, 5 and 6, 7 and 8, respectively) or 0.02, 0.08, 0.32, or 1.3 μg of GagΔp6 (lanes 9 and 10, 11 and 12, 13 and 14, and 15 and 16, respectively) at 0° (odd-numbered lanes) or 37°C (even-numbered lanes) for 60 min. Lanes 17 to 19 represent markers, in which the labeled oligonucleotide was mixed with nothing (lane 17), with the 28-base complementary oligonucleotide alone (lane 18), or with the 21-base complementary oligonucleotide alone (lane 19), heated to 90°C for 5 min, and allowed to cool slowly to room temperature. (B) Data from incubations at 37°C in panel A as analyzed by phosphorimaging.