. 2023 May 17;16(5):1075–1083. doi: 10.14202/vetworld.2023.1075-1083

Table-2.

Velocity of spermatozoa (VCL, VSL, and VAP) liquid semen with various diluent formulas.

VCL	Days of cold storage

	0	1	2	3	4	5
T1	74.6 ± 6.7^abc	62.6 ± 15.3^b	42.5 ± 14.0^ab	40.4 ± 18.1^bc	24.1 ± 7.3^ab	22.4 ± 9.6^b
T2	76.3 ± 6.5^abcd	70.6 ± 9.6^bc	58 ± 10.9^cd	53.8 ± 12.6^de	44.5 ± 8.7^d	33.6 ± 11.4^cd
T3	81.7 ± 10.0^cd	76.7 ± 7.9^cd	68.2 ± 6.8^def	59.5 ± 12.7^e	50 ± 16.5^de	51 ± 9.7^ef
T4	82.5 ± 9.9^d	79.9 ± 7.3^d	75.3 ± 9.4^f	70.5 ± 9.2^f	60.2 ± 11.9^ef	55.3 ± 12.0^f
T5	76.3 ± 7.7^abcd	83 ± 8.4^d	69.7 ± 17.9^ef	72.6 ± 10.1^f	66 ± 10.6^f	58.6 ± 12.4^f
T6	74.1 ± 1.8^ab	52.4 ± 6.4^a	33.6 ± 15.1^a	24.5 ± 14.7^a	19.3 ± 11.9^a	8.6 ± 6.6^a
T7	76 ± 6.1^abcd	63.3 ± 8.1^b	49.4 ± 7.1^bc	36.7 ± 14.1^b	25.7 ± 9.7^ab	19.1 ± 12.1^b
T8	76.1 ± 5.5^abcd	65.7 ± 3.8^b	53.6 ± 5.7^c	42.9 ± 9.0^bcd	31.6 ± 10.4^bc	28.7 ± 8.1^b
T9	72.5 ± 6.3^a	67.4 ± 6.6^b	54.4 ± 9.9^c	54.4 ± 6.0^de	40.3 ± 13.1^cd	33.1 ± 8.8^cd
T10	72.4 ± 6.5^a	67.9 ± 6.9^b	60.2 ± 5.8^cde	51 ± 7.0^cde	43.9 ± 11.3^d	42.3 ± 10.7^de
T11	80.9 ± 4.2^bcd	90.9 ± 8.1^e	77.8 ± 10.4^f	72.5 ± 9.0^f	67 ± 7.3^f	57.6 ± 5.0^f
VSL
T1	37.9 ± 7.1^abc	26.3 ± 6.4^ab	17.9 ± 8.2^ab	13.6 ± 7.7^ab	8.9 ± 4.3^ab	6.6 ± 3.8^ab
T2	35.2 ± 6.5^ab	27.9 ± 3.7^abc	22.6 ± 5.5^bc	19.8 ± 4.5^cd	14.8 ± 3.6^cde	11.7 ± 5.1^bcd
T3	34.2 ± 5.0^ab	28.6 ± 7.3^abc	25.9 ± 3.3^cd	20.3 ± 2.6^cd	14.7 ± 4.2^cde	14.6 ± 2.7^cd
T4	34.1 ± 5.2^ab	27.6 ± 4.9^abc	24.9 ± 2.6^c	21.1 ± 2.8^cd	18 ± 1.5^def	16.1 ± 2.3^cde
T5	31.5 ± 4.1^a	27.1 ± 3.9^abc	22.5 ± 1.8^bc	21 ± 2.1^cd	18.9 ± 1.7^ef	16.9 ± 2.8^de
T6	41.6 ± 5.6^bcd	25.6 ± 5.6^a	15.1 ± 7.8^a	9.8 ± 7.2^a	6 ± 5.4^a	3.4 ± 3.0^a
T7	47.6 ± 7.4^d	32.2 ± 6.3^cd	24 ± 5.7^c	17.3 ± 9.6^bc	10 ± 4.9^abc	6.6 ± 7.1^ab
T8	47.6 ± 9.6^d	31.5 ± 5.1^bcd	27.4 ± 4.7^cd	20.6 ± 6.9^cd	13.2 ± 6.3^bcd	11.2 ± 5.0^bc
T9	41.9 ± 9.4^bcd	34.9 ± 6.4^d	26.4 ± 5.8^cd	27.8 ± 3.4^e	19.6 ± 7.4^ef	14.1 ± 7.0^cd
T10	43.3 ± 10.9^cd	32.5 ± 5.7^cd	30.9 ± 5.6^d	25.7 ± 5.5^de	20.7 ± 6.1^fg	21.2 ± 7.2^e
T11	41.9 ± 11.3^bcd	25.5 ± 2.4^a	26.2 ± 4.1^cd	24.5 ± 2.5^de	25 ± 3.0^g	21 ± 2.5^e
VAP
T1	51.8 ± 5.8^a	40.1 ± 8.3^ab	28.8 ± 12.3^b	23.4 ± 12.3^b	14 ± 7.1^ab	11.7 ± 7.0^abc
T2	52.6 ± 5.1^a	47 ± 6.0^cd	38.2 ± 8.5^cde	35.3 ± 9.1^cde	28.6 ± 7.2^d	20.4 ± 8.0^d
T3	53.9 ± 6.9^ab	48.6 ± 7.7^cd	45.2 ± 5.4^ef	37.5 ± 7.3^de	29.7 ± 10.5^d	32.4 ± 8.9^ef
T4	54.4 ± 7.7^ab	48 ± 6.5^cd	46.6 ± 6.0^f	42.1 ± 3.8^e	37.1 ± 5.4^e	33.3 ± 6.8^ef
T5	49 ± 3.5^a	48.3 ± 4.6^cd	43.2 ± 5.4^def	42.7 ± 4.6^e	39.1 ± 4.7^e	34.5 ± 6.3^ef
T6	55.8 ± 4.6^ab	35.5 ± 6.4^a	21.5 ± 10.5^a	14.7 ± 10.1^a	10.5 ± 6.1^a	4.9 ± 3.8^a
T7	60.5 ± 7.0^b	42.5 ± 6.9^bc	34 ± 6.0^bc	23.4 ± 11.4^b	14.1 ± 6.1^ab	11.2 ± 10.1^ab
T8	60.4 ± 9.3^b	42.2 ± 5.1^bc	36.2 ± 4.9^cd	27.8 ± 8.7^bc	18.5 ± 8.1^bc	15.7 ± 6.3^bcd
T9	55.1 ± 8.2^ab	45.2 ± 7.0^bcd	35.2 ± 7.1^bc	36.6 ± 4.6^de	25.3 ± 9.3^cd	19.2 ± 6.7^cd
T10	56.1 ± 10.4^ab	43.9 ± 4.5^bc	40 ± 5.6^cdef	33 ± 5.8^cd	27.7 ± 7.9^d	27.7 ± 8.4^e
T11	61.1 ± 8.6^b	50.7 ± 4.0^d	47 ± 3.3^f	42.9 ± 4.2^e	41.9 ± 5.4^e	38 ± 4.0^f

Different letter notations in the same column indicate a significant difference (p < 0.05). VCL=Velocity curve linear, VSL=Velocity straight linear, VAP=Velocity average pathway