. 2023 May 17;16(5):1075–1083. doi: 10.14202/vetworld.2023.1075-1083

Table-4.

Amplitude lateral head, BCF, and H of spermatozoa liquid semen with various diluent formulas.

ALH	Days of cold storage

	0	1	2	3	4	5
T1	2.8 ± 0.4^bc	2.6 ± 0.4^abc	2.1 ± 0.7^ab	1.9 ± 0.7^b	0.5 ± 0.3^a	0.9 ± 1.0^a
T2	3 ± 0.4^cd	2.8 ± 0.4^c	2.5 ± 0.3^cde	2.4 ± 0.3^bcd	2.1 ± 0.7^bc	1.7 ± 0.9^b
T3	3.3 ± 0.3^d	3 ± 0.2^d	2.8 ± 0.4^def	2.6 ± 0.1^cde	2.4 ± 0.5^bc	1.8 ± 1.0^b
T4	3.3 ± 0.3^d	3.3 ± 0.4^ef	3.1 ± 0.4^fg	2.8 ± 0.4^de	2.7 ± 0.6^c	2.4 ± 0.4^bc
T5	3.2 ± 0.4^d	3.4 ± 0.3^f	3.3 ± 0.3g	3 ± 0.4^e	2.7 ± 0.4^c	2.6 ± 0.5^c
T6	2.5 ± 0.3^ab	2.4 ± 0.3^a	2 ± 0.7^a	1.3 ± 0.9^a	0.7 ± 0.7^a	0.2 ± 0.6^a
T7	2.3 ± 0.3^a	2.5 ± 0.2^ab	2.1 ± 0.3^ab	2.1 ± 0.8^bc	1.8 ± 1.0^b	0.4 ± 0.6^a
T8	2.3 ± 0.5^a	2.6 ± 0.2^abc	2.3 ± 0.2^abc	2.2 ± 0.2^bc	1.8 ± 0.6^b	1.7 ± 0.7^b
T9	2.4 ± 0.4^a	2.6 ± 0.2^abc	2.4 ± 0.2^bcde	2.3 ± 0.4^bc	2.1 ± 0.9^bc	2.2 ± 0.7^bc
T10	2.3 ± 0.4^a	2.7 ± 0.2^bc	2.4 ± 0.3^bcd	2.3 ± 0.2^bc	2.1 ± 0.5^bc	2 ± 0.5^bc
T11	2.4 ± 0.4^a	3.2 ± 0.2^de	2.8 ± 0.2^ef	2.8 ± 0.2^de	2.7 ± 0.4^c	2.2 ± 0.3^bc
BCF
T1	9.6 ± 1.1^b	10.9 ± 1.5^bcde	8.3 ± 2.6^ab	8.9 ± 4.4^b	3.3 ± 2.0^a	2.7 ± 3.2^a
T2	8.3 ± 1.5^ab	10.5 ± 1.2^bcd	9.5 ± 1.5^abc	9.5 ± 1.2^bc	7.8 ± 2.3^bc	6.8 ± 3.6^b
T3	8.4 ± 1.5^ab	9.9 ± 1.4^ab	9.6 ± 2.1^abc	9.6 ± 1.2^bcd	8.3 ± 1.0^bcd	7.2 ± 3.9^b
T4	8.1 ± 1.4^ab	9 ± 1.5^a	9.2 ± 1.6^ab	8.6 ± 1.0^b	8.8 ± 1.6^bcd	8.4 ± 1.3^bc
T5	7.8 ± 1.7^a	9.1 ± 1.1^a	8 ± 1.3^a	8.5 ± 1.2^b	8.5 ± 0.7^bcd	8.5 ± 1.3^bc
T6	9.1 ± 1.5^ab	10.9 ± 0.8^bcde	9.2 ± 3.0^ab	6.3 ± 4.3^a	1.9 ± 2.4^a	1.2 ± 3.0^a
T7	9.2 ± 1.8^ab	12 ± 1.2^e	11.2 ± 1.5^cde	9.1 ± 3.9^b	7.4 ± 3.5^b	2.5 ± 3.0^a
T8	9.5 ± 2.0^ab	11.7 ± 1.3^de	11.5 ± 1.4^de	11.5 ± 1.1^cde	9 ± 3.7^bcd	7.9 ± 3.9^b
T9	8.7 ± 2.0^bc	11.6 ± 1.6^de	11.4 ± 1.1^de	12.2 ± 0.8^e	10.5 ± 4.0^cd	8.9 ± 3.1^bc
T10	8.8 ± 1.7^ab	11.4 ± 1.6^cde	12.2 ± 1.3^e	11.9 ± 0.8^de	11.1 ± 2.6^d	11.5 ± 3.4^c
T11	7.8 ± 1.6^a	10 ± 1.0^abc	9.8 ± 1.2^bcd	10 ± 0.9^bcde	9.8 ± 0.6^bcd	9.5 ± 1.0^bc
H
T1	23.5 ± 6.6^cd	9.7 ± 6.2^ab	2.7 ± 1.8^ab	1.5 ± 1.6^a	0.1 ± 0.2^a	1.1 ± 1.4^a
T2	21.1 ± 3.9^bc	9.8 ± 3.1^ab	6.9 ± 3.6^abc	2.9 ± 2.6^a	1.7 ± 2.0^a	1.3 ± 0.9^a
T3	27 ± 9.0^cd	17.2 ± 5.9^de	11 ± 4.3^cde	6.7 ± 2.6^b	4.5 ± 3.1^a	0.9 ± 0.7^a
T4	26.8 ± 10.4^cd	19.6 ± 5.5^de	15.8 ± 6.8^ef	11.6 ± 3.4^c	9.7 ± 7.2^b	5.7 ± 3.0^b
T5	28.4 ± 4.7^d	20.8 ± 5.7^e	18.3 ± 8.7^f	14.9 ± 5.6^d	13.8 ± 8.1^c	10.6 ± 8.0^c
T6	15.7 ± 3.2^ab	6.1 ± 3.9^a	1.9 ± 2.2^a	0.4 ± 0.6^a	0.1 ± 0.2^a	0.2 ± 0.5^a
T7	15.3 ± 5.9^ab	11.6 ± 5.1^bc	3.3 ± 2.4^abc	2.2 ± 2.0^a	0.4 ± 0.5^a	0.1 ± 0.1^a
T8	14 ± 5.5^a	15 ± 1.8^cd	7.1 ± 2.9^bc	1.7 ± 1.1^a	0.5 ± 0.6^a	0.6 ± 1.0^a
T9	14.6 ± 5.0^a	17.7 ± 5.6^de	10.3 ± 5.9^cd	6.3 ± 5.0^b	2.4 ± 2.2^a	1.1 ± 1.6^a
T10	14.8 ± 4.8^a	17.3 ± 4.8^de	12.7 ± 7.1^de	7.3 ± 3.9^b	3.4 ± 3.2^a	1.3 ± 1.6^a
T11	12.8 ± 4.6^a	11.7 ± 2.5^bc	12 ± 2.4^cde	10.9 ± 1.4^c	9.5 ± 2.4^b	5.3 ± 2.9^b

Different letter notations in the same column indicate a significant difference (p < 0.05). ALH=Amplitude lateral head, BCF=Beat cross frequency, H=Hyperactivity