. 2023 May 17;16(5):1075–1083. doi: 10.14202/vetworld.2023.1075-1083

Table-5.

Viability and abnormality of spermatozoa liquid semen with various diluent formulas.

Viability	Days of cold storage

	0	1	2	3	4	5
T1	85.4 ± 5.2^b	75 ± 11.6^ab	66 ± 9.2^ab	65.4 ± 20.5^abc	49.6 ± 19.1^a	33.8 ± 17.3^a
T2	85.8 ± 6.1^b	84.4 ± 10.4^b	81 ± 10.6^cd	70 ± 14.6^abc	66 ± 11.6^a	54.6 ± 9.1^bc
T3	90 ± 4.1^b	86 ± 5.5^b	86.4 ± 4.2^cd	84 ± 4.3^c	76.8 ± 5.5^bc	71 ± 10.0^cd
T4	85.8 ± 4.0^b	87.4 ± 7.4^b	81.4 ± 7.8^cd	83.2 ± 4.6^c	81.6 ± 8.0^bc	81.8 ± 3.5^d
T5	85.4 ± 6.9^b	86.2 ± 8.8^b	90.4 ± 6.7^d	73.6 ± 17.8^abc	87.6 ± 4.0^c	86.4 ± 2.6^d
T6	72.2 ± 19.0^a	68 ± 12.5^a	63.8 ± 13.9^a	57.2 ± 13.0^ab	47.6 ± 19.1^a	37.6 ± 16.5^a
T7	80 ± 8.5^ab	77 ± 16.3^ab	72.8 ± 14.3^abc	53.4 ± 27.1^a	50.4 ± 26.6^a	46.4 ± 24.4^ab
T8	83.2 ± 11.1^ab	79.8 ± 11.3^ab	78.2 ± 12.0^bcd	74.2 ± 6.8^abc	71.2 ± 10.1^bc	55.2 ± 20.8^bc
T9	85.2 ± 6.2^b	84.8 ± 6.1^b	80.2 ± 6.5^cd	76.6 ± 11.4^bc	64.6 ± 15.2^ab	57.8 ± 19.3^bc
T10	83.8 ± 7.8^ab	88.2 ± 6.1^b	86.4 ± 5.6^cd	78.8 ± 9.7^bc	77 ± 7.7^bc	71.6 ± 13.8^cd
T11	90.8 ± 6.4^b	86.8 ± 13.3^b	88.8 ± 5.8^d	87 ± 6.2^c	87.8 ± 3.0^c	83.8 ± 1.7^d
Abnormality
T1	1.6 ± 0.9	0.8 ± 0.8	0.8 ± 1.3	0.8 ± 0.8	1.4 ± 1.3	0.6 ± 0.9^ab
T2	1.2 ± 0.8	1 ± 1.7	0.8 ± 0.8	1.4 ± 1.1	1.2 ± 2.2	1.4 ± 1.1^ab
T3	1.2 ± 0.8	1.4 ± 1.5	0.2 ± 0.4	0.8 ± 0.8	1 ± 1.2	1.2 ± 1.1^ab
T4	2 ± 0.7	1.2 ± 0.8	1.8 ± 2.9	1.6 ± 1.3	1.6 ± 1.5	1.4 ± 1.7^ab
T5	1.2 ± 1.8	1.8 ± 0.8	0.8 ± 0.8	1.8 ± 1.6	1.6 ± 1.9	1 ± 1.0^ab
T6	1.6 ± 1.1	1.4 ± 1.1	0.8 ± 1.1	0.8 ± 0.8	0.6 ± 0.5	0.2 ± 0.4^a
T7	2 ± 1.4	1.8 ± 1.3	1 ± 1.4	1.2 ± 0.8	2.2 ± 2.2	0.4 ± 0.5^ab
T8	1 ± 1	1.8 ± 2.4	1.2 ± 1.8	1 ± 1.2	1 ± 1.2	1.6 ± 0.9^ab
T9	1.2 ± 0.8	1.6 ± 1.7	1 ± 1.4	0.8 ± 1.1	1.4 ± 1.1	2.2 ± 1.9^b
T10	1.8 ± 2.7	1.4 ± 0.5	0.6 ± 0.9	0.6 ± 0.9	1.8 ± 1.3	2 ± 1.6^ab
T11	1.8 ± 2.2	1.5 ± 1	0.5 ± 1	1.3 ± 1.2	2.5 ± 1	1.8 ± 1.3^ab

Different letter notations in the same column indicate a significant difference (p < 0.05)