FIG. 3.

Numbers of MHV-68-specific CD4⁺ T cells generated in the acute and persistent phases of the host response. B6 mice were infected i.n. with 600 PFU of MHV-68, and CLN, MLN, and spleen cells from individual animals were incubated for 48 h in the ELISpot assay. The numbers of MHV-68-specific CD4⁺ T cells in each organ were determined from the frequency, the percentage of CD4⁺ T cells, and total cell count. Each data point represents an individual mouse. The results were compiled from four experiments, indicated by the different symbols.