Figure 6.

Enzymatic hydrolysis of B. gasipaes inner sheaths and peel with and without biological pretreatment. The incubation system (10 mL) contained 50 mmol/L citrate buffer (pH 5.0) and 0.5 g of lignocellulosic materials. Incubation was performed in Erlenmeyer flasks at 37 °C and under agitation (150 rpm). Aliquots of 0.3 mL were taken periodically for the determination of total reducing sugars (TRS) by means of the 3,5-dinitrosalicylic acid method and glucose by means of the glucose oxidase-peroxidase method. The experimental points are the means plus mean standard errors of 3 experiments.