FIG. 4.

TRS specificity. In vitro-translated AAV2 Rep78 or AAV5 Rep78 was incubated with radiolabeled probe (50,000 cpm), and the products were resolved on denaturing gels. The positions of the full-length substrate and cleavage products are indicated by arrows. Lanes: 1 and 2, probe alone; 3 and 4, AAV5 Rep78 with the AAV5 and AAV2 ITRs, respectively; 5 and 6, AAV2 Rep78 with the AAV5 and AAV2 ITRs, respectively. The upper arrow identifies the AAV5 cleavage product (lane 3), and the lower arrow indicates the AAV2 cleavage product (lane 6).