Figure 5.

Photomicrograph from the tumor mass of different experimental groups immune-stained with P⁵³. (GII): cells with negative staining reaction. A few apoptotic tumor cells appeared with dark positive cytoplasmic staining reaction (blue stars); (GIII): large numbers of necrotic cells with hazard staining reaction to the used marker. Some cells showed apoptotic reaction with dark positive brownish nuclear staining (dark circle); (GIV): marked apoptotic reaction in about 75–80% of the tumor cells as represented by dense brown cytoplasmic stainability of the affected tumor cells (blue stars); (GV): marked apoptotic reaction in about 75–80% of the tumor cells as represented by dense brown cytoplasmic stainability of the affected tumor cells (blue stars).