FIG. 3.

Autoradiographic images of electrophoretically separated BamHI digests of recombinant viral DNA hybridized with ³²P-labeled pRB5210. The digests were electrophoretically separated in a 0.8% agarose gel, transferred to Zeta-probe membrane, and hybridized with ³²P-labeled pRB5210 carrying the HSV-1(F) BamHI N fragment. Lanes: 1, the 4.9-kb BamHI N fragment from HSV-1(F); 2, the 4.9-kb BamHI N fragment from the R7905 [HSV-1(F)] cosmid virus; 3, the 3.6-kb BamHI N fragment from R7802 recombinant virus; 4, the 3.4-kb BamHI N fragment from the R7805 recombinant virus.