FIG. 5.
Photograph of an immunoblot of electrophoretically separated lysates of cells mock infected or infected with HSV-1(F) or R7356 (ΔUL13) and reacted with antibody to ICP22/US1.5. Vero cells (VC) and rabbit skin cells (RSC) harvested at various times (4 to 24 h) after infection were solubilized, subjected to electrophoresis in a denaturing 10% polyacrylamide gel, transferred to a nitrocellulose sheet, and reacted with the rabbit polyclonal antibody (W2) made against the 138 carboxyl-terminal amino acids of ICP22/US1.5.