FIG. 8.

Photograph of an immunoblot of electrophoretically separated lysates of cells infected with R7815, R7805, or R7808 and reacted with the polyclonal antibody W2 to ICP22/U_S1.5. Vero cells harvested at 18 h after infection were solubilized, subjected to electrophoresis in a denaturing 10% polyacrylamide gel, transferred to a nitrocellulose sheet, and reacted with the polyclonal antibody prepared against the carboxyl-terminal amino acids of ICP22/U_S1.5. Lanes: 1, R7815 (Δα22_NT/U_S1.5)[Δ47a.a.]; 2, R7805 (Δα22/U_S1.5); 3, R7808 (Δα22/U_S1.5). The numbers next to the arrows indicate the initiator methionine for the unprocessed protein product in each lane.