FIG. 9.
Photograph of an immunoblot of electrophoretically separated lysates of cells mock-infected or infected with HSV-1(F), R7802, R7804, R7805, R7806, R7808, or R7828 and reacted with antibodies to UL38 and US11. Replicate cultures of Vero cells (VC) (odd-numbered lanes) or rabbit skin cells (RSC) (even-numbered lanes) harvested at 18 h after infection were solubilized, subjected to electrophoresis in a denaturing polyacrylamide gel, transferred to a nitrocellulose sheet, then sequentially reacted with the monoclonal antibody to US11, and the polyclonal antibody to UL38. Lanes: 1 and 2, HSV-1(F); 3 and 4, R7802 (Δα22/ΔUS1.5); 5 and 6, R7804 (R7802 repair); 7 and 8, R7805 (Δα22NT/US1.5); 9 and 10, R7806 (R7805 repair); 11 and 12, R7808 (Δα22NT/US1.5α22p); 13 and 14, R7828 (R7808 repair).