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. 2023 Aug 4;12(15):2870. doi: 10.3390/plants12152870

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(a) TLC profile of the column chromatographic fractions (1–58) under UV (254 nm; A–E) and after spraying with H₂SO₄/vanillin and then being heated (F–J). TLC plate (A,F) consisted of fractions 1–15 (ref. to total extract FR) developed using ss A. TLC plate (B,G) consisted of fractions 15–28 (ref. to total extract FR) developed using ss A. TLC plate (C,H) consisted of fractions 28–39 (ref. to total extract FR) developed using solvent system B. TLC plate (D,I) consisted of fractions 39–52 (ref. to total extract FR) developed using solvent system C. TLC plate (E,J) consisted of fractions 52–61 (ref. to total extract FR) developed using solvent system D. FR refers to methanol total extract of H. rutilans. (b) TLC profile of the main fractions (I–XVI) under UV (254 nm; (K) and after spraying with H₂SO₄/vanillin and gentle heating (L). (c) TLC of X when sprayed with H₂SO₄/vanillin and then heated (M). TLC profile of XC after spraying with H₂SO₄/vanillin and heating (N). TLC profile of VIII after spraying with H₂SO₄/vanillin and heating (O). TLC profile of VI after spraying with H₂SO₄/vanillin and heating (P). (d) HPLC spectrum of C-2. (e) HPLC spectrum of C-1. (f) HPLC spectrum of C-3 and C-4.

(a) TLC profile of the column chromatographic fractions (1–58) under UV (254 nm; A–E) and after spraying with H₂SO₄/vanillin and then being heated (F–J). TLC plate (A,F) consisted of fractions 1–15 (ref. to total extract FR) developed using ss A. TLC plate (B,G) consisted of fractions 15–28 (ref. to total extract FR) developed using ss A. TLC plate (C,H) consisted of fractions 28–39 (ref. to total extract FR) developed using solvent system B. TLC plate (D,I) consisted of fractions 39–52 (ref. to total extract FR) developed using solvent system C. TLC plate (E,J) consisted of fractions 52–61 (ref. to total extract FR) developed using solvent system D. FR refers to methanol total extract of H. rutilans. (b) TLC profile of the main fractions (I–XVI) under UV (254 nm; (K) and after spraying with H₂SO₄/vanillin and gentle heating (L). (c) TLC of X when sprayed with H₂SO₄/vanillin and then heated (M). TLC profile of XC after spraying with H₂SO₄/vanillin and heating (N). TLC profile of VIII after spraying with H₂SO₄/vanillin and heating (O). TLC profile of VI after spraying with H₂SO₄/vanillin and heating (P). (d) HPLC spectrum of C-2. (e) HPLC spectrum of C-1. (f) HPLC spectrum of C-3 and C-4.