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Reporter plasmids. (a) Plasmid pDM128 (17, 18), which serves as a positive control and contains the wild-type RRE within an HIV-1 intron adjacent to a CAT reporter gene. SD, splice donor; SA, splice acceptor; LTR, long terminal repeat. (b) Insertion of RBE concatemers into pDM138. Plasmid pDM138 (21), a derivative of pDM128, contains a unique ClaI site in place of the RRE. RBE concatemers T1, T2, T3, T4, and T5 were inserted into the ClaI site of pDM138.
