Analysis of age effect on the observed EtOH dose-dependent LCN-related FC changes. (A) The three models use EtOH dose, as independent variables, sex and age as moderators, and three network-level FC changes as dependent variables: (top) within LCN, (middle) between LCN and DMN, and (bottom) between LCN and SN. In all three models, main effect of alcohol dose on LCN-related FC was dependent on age (a significant interaction effect, all p < 0.05) but not sex (all p > 0.05). (B) Dose-dependent increase in LCN-related FC in P45 and P80 groups of rats. Circles indicate data for individual subjects color-coded according to age group. Red and blue lines indicate linear regression slopes.