Fig. 5. RTFs.
a, Left, the ZFs of KLF6 are seamlessly replaced by designed ZFs. The consensus ZF motif, listed below, is used to guide the seamless replacement of parent ZFs. Right, sequence of the KLF6 TF and precise location of ZF replacement. b, A GFP reporter is activated with four ZF arrays designed to bind the tetO sequence. Array 3 is used to show that TFs other than KLF6 can also be reprogrammed to bind the tetO sequence and regulate the target. c, A GFP reporter is repressed by ZIM3 reprogrammed with tetO-binding array 3. This array can also be used to reprogram other repressing TFs in addition to ZIM3. d, Relative expression of CDKN1C by KLF6 reprogrammed with seven ZF arrays designed to bind sequences upstream of the TSS. e, Relative expression of DPH1 repressed by ZIM3 reprogrammed with 11 ZF arrays designed to bind sequences downstream of the TSS.