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. 2023 Jan 19;41(8):1089–1098. doi: 10.1038/s41587-022-01584-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — The activity of the supernatants from M. pneumoniae strains against biofilms of the indicated P. aeruginosa strains (SAT290, PAO1, Boston 41501, NCTC3437) was assessed by Crystal Volet assay. Briefly, Pseudomonas biofilms were generated by seeding in 96-well plates and incubating at 37 °C for 24 h. Biofilms were then treated at 37 °C for 4 h with M. pneumoniae supernatants to allow the activity of the dispersal enzymes. More details are given in Methods. a,b, Biofilm dispersal activity of the supernatants of M. pneumoniae strains expressing the indicated heterologous proteins, tested against biofilms of SAT290 (a) or PAO1 (b) strains. c, Biofilm dispersal activity of the supernatants of CV2 and CV2_HA. Data are shown as the mean of three independent experiments ± s.d. ^*P < 0.05, ^**P < 0.01 by two-sided Student’s t-test compared with the control strain. For details (for example, inoculum, growth and time), see Methods.