Fig. 3. Biofilm dispersion and antimicrobial activities of the CV2_HA_P1 strain in vitro.

a, Biofilm dispersal activity of the supernatant of strains CV2 (dashed lines) or CV2_HA_P1 (solid lines), assessed by Crystal Violet assay. Pseudomonas biofilms were generated in 96-well plates at 37 °C for 24 h and then treated at 37 °C with the indicated supernatants. Data are shown as the mean of three independent experiments ± s.d. with three technical replicates at each timepoint. b, Growth curves of different P. aeruginosa strains treated with supernatants from CV2 or CV2_HA_P1. Absorbance of the culture (OD₆₀₀) was measured every 20 min with a TECAN reader over 24 h. Errors bars indicate the s.d. of four replicates. For details (for example, inoculum, growth and time), see Methods.