Fig. 3.
MST1 downregulates SIRT3 through sirt3 promoter heterochromatinization, a SIRT3 mRNA expression profile in cardiomyocytes infected 48 h with an adenovirus overexpressing LacZ or MST1. Data represent mean ± SEM (n = 8 independent samples); b–c Pser14-H2B and H2B expression profiles after 4 h of treatment with 50 μM of doxorubicin in primary cardiomyocyte cultures infected with ad-LacZ or ad-DN-MST1 for 48 h. Densitometric data normalized by loading control represent mean ± SEM (n = 6 independent samples); d–e sirt3 promoter DNA amplification after chromatin immunoprecipitation (ChIP) targeting trimethyl (Lys27) Histone 3 (H3K27) in H9C2 cardiac cells infected 48 h with an adenovirus overexpressing LacZ or DN-MST1. Data represent mean ± SEM (n = 7 independent replicates from pulled cells); f SIRT3 mRNA expression profile in H9C2 cardiac cells infected 48 h with an adenovirus overexpressing LacZ or DN-MST1. Data represent mean ± SEM (n = 5–6 independent samples). Data were analysed with a two-tailed Student’s t-test (a) or one-way ANOVA with Bonferroni post-hoc test (b–f). *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001