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Steady-state localization of Us9-EGFP. Monolayers of Us9-EGFP-expressing cells grown on glass coverslips were treated with cycloheximide (50 μg/ml) for 0 h (A), 2 h (B), 4 h (C), and 6 h (D). At the indicated time points, the cells were fixed with paraformaldehyde and the localization of Us9-EGFP was visualized by fluorescence microscopy under UV illumination.
