FIG. 8.

Two determinants in the Us9 cytoplasmic tail are required for efficient internalization of Us9 from the plasma membrane. An indirect immunofluorescence assay was performed on PK15 cells stably expressing Us9-EGFP (PK15-BB14), Us9(d46-55)-EGFP (PK15-AB35), or Us9(L30-31A)-EGFP (PK15-AB37) essentially as described in the legend to Fig. 4. The temperature shift in this experiment was to 37°C. The composite shows only the red fluorescence of the Alexa 568-conjugated goat anti-rabbit IgG secondary antibody. Times are indicated in minutes.