FIG. 9.

Us9(d46-55) is efficiently incorporated into viral particles. Monolayers of PK15 cells were infected at a multiplicity of infection of 10 with either PRV Be or PRV 162 for 15 h. Cellular extracts were prepared, and virions were isolated from the medium by centrifugation through a 30% sucrose cushion. Both the cellular extracts and virions were fractionated on an SDS–12.5% polyacrylamide gel and analyzed by Western blotting with Us9 antiserum.