Table 3.
Summary of purified AfKatG enzyme from different fractions of bacterial culture using affinity chromatography. IP – intracellular protein (without PBP5/6 overexpression); IP PBP5/6 – intracellular protein (with PBP5/6 overexpression); EP – extracellular protein (without PBP5/6 overexpression); EP PBP5/6 – extracellular protein (with PBP5/6 overexpression); the symbol “±” signifies the standard deviation associated with a measurement of three independent biological replicates
| AfKatG | ||||
|---|---|---|---|---|
| IP | IP PBP5/6 | EP | EP PBP5/6 | |
| Protein yield (mg/L) | 342.8 ± 34.3 | 293.5 ± 3.9 | 3.2 ± 1.3 | 117.9 ± 56.0 |
| Enzyme-specific peroxidase activity (U/mg) | 5.2 ± 1.8 | 4.5 ± 0.8 | - | 3.9 ± 1.1 |
| Enzyme-specific catalase activity (U/mg) | 1865 ± 203 | 1766.2 ± 155.7 | - | 1358.3 ± 247.2 |