FIG. 2.

EBV and HVP EBNA-1 bind to human metaphase chromosomes. HeLa cells transfected with plasmids encoding EGFP (a), EGFP EBV EBNA-1 (b), or EGFP HVP EBNA-1 (c) were treated with colcemid, stained with Hoechst 33342, swollen in hypotonic buffer, centrifuged onto microscope slides, and observed in the presence of PBS either at 488 nm (EGFP) (a to c) or at 365 nm (Hoechst) (d to f). Whereas EGFP was localized in the nucleoplasm with frequent chromosome exclusion, EGFP EBV EBNA-1 and EGFP HVP EBNA-1 specifically bound to metaphase chromosomes.