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. 1999 May;73(5):4385–4392. doi: 10.1128/jvi.73.5.4385-4392.1999

FIG. 5.

FIG. 5

FIG. 5

Mapping of CBS-1 following metaphase spreadings. (A) Various derivatives of EBV and HVP EBNA-1 were tested for their ability to interact with metaphase chromosomes following transfection and chromosome spreading on slides. Mutants M10 (a) and M11 (c) associated with metaphase chromosomes, whereas Rand1 (d) and Rand2 (e), two mutants containing random combinations of M11 amino acids, did not. EGFP-HVP.67-97 (b) encoded the HVP region homologous to amino acids 70 to 89 of EBV EBNA-1. The same cells were observed at 365 nm (Hoechst) (f to j). (B) Amino acids (aa) 70 to 89 of EBV EBNA-1 and amino acids 67 to 93 of HVP EBNA-1 contain a short stretch of highly homologous amino acids (amino acids 72 to 84). Three mutants containing amino acids 72 to 84 (M11) or random combinations of these amino acids (Rand1 and Rand2) were constructed and tested for their ability to interact with metaphase chromosomes.