Figure 6.

C5ar1 deficiency prevented bone loss induced by loss of estrogens

(A) μ-CT quantitative parameters and 3D representation of cortical bone tibiae from an estrogen deficiency model by performing sham or ovariectomy (OVX) surgery on WT or C5aR1^−/− female mice. n = 9–13. (B) Quantitative parameters and 3D representation of the trabecular compartment of tibiae. n = 9–13. (C) Quantification of the number of osteoblasts per bone surface (osteoblasts/mm) and osteocytes per cortical bone area (osteocytes/mm²) from H&E-stained sections of tibiae. The number of osteoclasts per trabecular bone surface (osteoclasts/mm) was quantified from TRAP staining of tibiae. n = 4–5. (D) Expression of osteoblastic (Runx2, Osx, and Col1a1) and osteoclastic (Nfatc1, Mmp9, Ctsk, and Trap) markers. RNA isolated from the tibiae and femurs of sham or OVX WT and C5aR1^−/− mice. n = 6–9. Data shown as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. One-way ANOVA.