TABLE 2.

Summary of CD and sedimentation equilibrium data for the I573 mutants

Peptide	[θ]222 values (10−3)a	Tm (°C)b	Molecular mass (kDa)c
N34(L6)C28	−31	70	24.4
I573L	−31	67	25.1
I573V	−31	65	24.9
I573A	−24	37	24.1
I573S	−13	25	NAd
I573P	−15	24	NA

^a[θ]₂₂₂ was measured at 0°C at monomer peptide concentrations of 10 μM. 

^bT_m was determined at monomer peptide concentrations of 10 μM. 

^cApparent molecular masses were determined with initial peptide concentrations of 10, 30, and 100 μM at 20°C. 

^dNA, not applicable. I573S and I573P each form an insoluble aggregate at concentrations above ∼10 μM in PBS.