Skip to main content
. 2023 Aug 7;15(15):3328. doi: 10.3390/polym15153328

Figure 15.

Figure 15

(Left: (AD)) Gallery of fluorescent control images presenting fibroblasts growing on a glass surface that were specifically labeled for actin microfilaments using phalloidin (red). The nuclear label (blue) was obtained via DAPI counterstaining. All cells reveal a properly stretched appearance, exhibiting strongly developed stress fibers (SF; AD). Note the prominent organization order of the stretched fibers in which their bundles form nearly parallel rows. Stars (B,C) indicate motile and properly stretched cells, both producing large lamellipodia. End-feet are outlined with dotted circles (A,C). The arrows in (C,D) indicate long primary processes with growing secondary processes. (Right: (AD)) Fluorescent images presenting pMSFs that grow on the surface of 1L6 LPP. The same labeling as in the case of fibroblasts growing on a glass surface is used. All cells were properly stretched (AD) and contained profound stress fibers (SF). The star in (B) marks a properly stretched cell that exhibits large lamellipodia. The end-feet are outlined with dotted circles (A,C). Long primary processes that produce secondary sprouts are marked with arrows (B). Note that in (C,D), very long and abundant primary processes emanating from different cells acquire complex network-like appearances by crossing each other and running on the surfaces of cells.