FIG. 2.

Single-stranded HMA of the env V1-V2 region. Genetic diversity in env V1-V2 was assessed by HMA with DNA extracted from infected PBMC (PB) (28 days postchallenge depicted for all; 100 days postchallenge depicted for macaques 1474, 1482, 1484, 1488, 1490, and 1510) or from lymph node (LN) (170 days postchallenge) or with plasma viral RNA (PL) (28 days postchallenge). (A) Animals 1472, 1474, 1476, and 1478 (5-week challenge group), animal 1480 (nonimmunized control), and animal 1510 (25-week challenge group); (B) macaques 1482, 1484, 1488, and 1490 (10-week challenge group) and macaque 1492 (nonimmunized control). S.S., single-stranded probe; H.D., homoduplex.