TABLE 4.
BAL cell types at days 3 and 6 after challenge with B1 or CP52 in mice vaccinated with formalin-inactivated virus
Treatmenta
|
Cell typeb | H&E staining
|
||||
---|---|---|---|---|---|---|
Day 3 p.i.
|
Day 6 p.i.
|
|||||
Immunization | Challenge | Range, % positive | Median % (Pc) | Range, % positive | Median % (P) | |
FI-B1 | B1 | MAC | 30–42 | 35 (<0.01) | 6–15 | 14 (<0.01) |
PMN | 2–8 | 6 (>0.01) | 3–8 | 6 (>0.10) | ||
EOS | 30–40 | 32 (<0.01) | 50–62 | 50 (<0.01) | ||
LYM | 25–30 | 27 (>0.05) | 25–38 | 30 (<0.05) | ||
FI-B1 | CP52 | MAC | 68–75 | 72 | 68–75 | 75 |
PMN | 2–9 | 5 | 5–11 | 6 | ||
EOS | 2–10 | 5 | 1–7 | 6 | ||
LYM | 15–22 | 18 | 10–18 | 13 | ||
FI-CP52 | B1 | MAC | 80–88 | 83 (>0.10) | 85–92 | 89 (>0.10) |
PMN | 0–5 | 2 (>0.10) | 1–6 | 4 (>0.10) | ||
EOS | 2–8 | 6 (>0.10) | 0–4 | 2 (>0.10) | ||
LYM | 5–11 | 9 (>0.10) | 3–10 | 5 (>0.10) | ||
FI-CP52 | CP52 | MAC | 78–85 | 81 | 87–96 | 94 |
PMN | 3–9 | 5 | 1–6 | 2 | ||
EOS | 0–6 | 2 | 0 | 0 | ||
LYM | 10–18 | 12 | 2–8 | 4 | ||
FI-PIV3 | B1 | MAC | 82–90 | 87 (>0.10) | 58–72 | 70 (>0.10) |
PMN | 1–6 | 3 (>0.10) | 11–18 | 11 (>0.10) | ||
EOS | 0–4 | 2 (>0.10) | 8–14 | 8 (>0.10) | ||
LYM | 6–12 | 8 (>0.10) | 6–12 | 11 (>0.10) | ||
FI-PIV3 | CP52 | MAC | 76–88 | 80 | 52–76 | 72 |
PMN | 2–6 | 6 | 6–11 | 10 | ||
EOS | 2–10 | 5 | 6–19 | 13 | ||
LYM | 6–14 | 9 | 6–18 | 5 |
Mice were i.n. infected with 104 PFU of virus or i.p. administered 104 PFU equivalents of formalin-inactivated virus.
BAL was collected and analyzed by H&E staining for percent MAC, PMN, EOS, and LYM.
The nonparametric Mann-Whitney analysis was used to test for significant differences in percent cells between B1- and CP52-infected mice within each grouping. Total cell numbers in BAL of B1- and CP52-infected mice ranged from 3 × 105 to 6 × 105/ml and from 3 × 105 to 6 × 105/ml, respectively.