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TABLE 4.

BAL cell types at days 3 and 6 after challenge with B1 or CP52 in mice vaccinated with formalin-inactivated virus

Treatmenta
Cell typeb H&E staining
Day 3 p.i.
Day 6 p.i.
Immunization Challenge Range, % positive Median % (Pc) Range, % positive Median % (P)
FI-B1 B1 MAC 30–42 35 (<0.01) 6–15 14 (<0.01)
PMN 2–8 6 (>0.01) 3–8 6 (>0.10)
EOS 30–40 32 (<0.01) 50–62 50 (<0.01)
LYM 25–30 27 (>0.05) 25–38 30 (<0.05)
FI-B1 CP52 MAC 68–75 72  68–75 75 
PMN 2–9 5  5–11 6 
EOS 2–10 5  1–7 6 
LYM 15–22 18  10–18 13 
FI-CP52 B1 MAC 80–88 83 (>0.10) 85–92 89 (>0.10)
PMN 0–5 2 (>0.10) 1–6 4 (>0.10)
EOS 2–8 6 (>0.10) 0–4 2 (>0.10)
LYM 5–11 9 (>0.10) 3–10 5 (>0.10)
FI-CP52 CP52 MAC 78–85 81  87–96 94 
PMN 3–9 5  1–6 2 
EOS 0–6 2  0 0 
LYM 10–18 12  2–8 4 
FI-PIV3 B1 MAC 82–90 87 (>0.10) 58–72 70 (>0.10)
PMN 1–6 3 (>0.10) 11–18 11 (>0.10)
EOS 0–4 2 (>0.10) 8–14 8 (>0.10)
LYM 6–12 8 (>0.10) 6–12 11 (>0.10)
FI-PIV3 CP52 MAC 76–88 80  52–76 72 
PMN 2–6 6  6–11 10 
EOS 2–10 5  6–19 13 
LYM 6–14 9  6–18 5 
a

Mice were i.n. infected with 104 PFU of virus or i.p. administered 104 PFU equivalents of formalin-inactivated virus. 

b

BAL was collected and analyzed by H&E staining for percent MAC, PMN, EOS, and LYM. 

c

The nonparametric Mann-Whitney analysis was used to test for significant differences in percent cells between B1- and CP52-infected mice within each grouping. Total cell numbers in BAL of B1- and CP52-infected mice ranged from 3 × 105 to 6 × 105/ml and from 3 × 105 to 6 × 105/ml, respectively. 

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