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TABLE 6.

BAL cell types at day 3 and 6 after challenge of control mice with B1 or CP52

Treatmenta
H&E staining
Flow cytometry
Cell typeb Day 3 p.i.
Day 6 p.i.
Cell phenotyped Day 3 p.i.
Day 6 p.i.
Immunization Challenge Range, % positive Median % (Pc) Range, % positive Median % (P) Range, % positive Median % (P) Range, % positive Median % (P)
pcDNA B1 MAC 85–92 88 (>0.10) 78–96 94 (>0.10) CD3 25–36 0 (>0.10) 33–42 35 (<0.01)
PMN 0–5 2 (>0.10) 1–5 3 (>0.10) B220 1–10 5 (>0.10) 1–16 8 (>0.10)
EOS 0–2 2 (>0.10) 0–2 1 (>0.10) DX5 0–8 5 (<0.01) 3–12 7 (<0.01)
LYM 5–11 8 (>0.10) 0–2 2 (>0.10) RB6-8C5 2–10 5 (<0.05) 1–9 6 (<0.01)
CD11b 8–15 10 (<0.01) 2–10 6 (>0.10)
pcDNA CP52 MAC 80–95 87  70–94 92  CD3 30–40 34  12–17 14 
PMN 1–5 3  0–12 3  B220 0–4 2  4–10 6 
EOS 0 0 1–6 1  DX5 30–38 35  37–54 48 
LYM 2–12 10  1–5 4  RB6-8C5 8–28 22  19–25 22 
CD11b 25–40 38  3–10 6 
a

Mice were i.n. infected with 104 PFU of virus or with pcDNA as described in Materials and Methods. 

b

BAL was collected and analyzed by H&E staining for percent positive MAC, PMN, EOS, and LYM. 

c

The nonparametric Mann-Whitney analysis was used to test for significant differences in percent cells between B1- and CP52-infected mice within each grouping. Total cell numbers in BAL of B1- and CP52-infected mice ranged from 2.0 × 105 to 3.5 × 105/ml and from 2.2 × 105 to 3.5 × 105/ml, respectively. 

d

BAL was collected and analyzed by flow cytometry for percent CD3+ T cells, B220+ B cells, DX5+ NK cells, RB6-8C5 neutrophils, and CD11b+ PMN. 

HHS Vulnerability Disclosure