TABLE 6.
BAL cell types at day 3 and 6 after challenge of control mice with B1 or CP52
Treatmenta
|
H&E staining
|
Flow cytometry
|
|||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Cell typeb | Day 3 p.i.
|
Day 6 p.i.
|
Cell phenotyped | Day 3 p.i.
|
Day 6 p.i.
|
||||||
Immunization | Challenge | Range, % positive | Median % (Pc) | Range, % positive | Median % (P) | Range, % positive | Median % (P) | Range, % positive | Median % (P) | ||
pcDNA | B1 | MAC | 85–92 | 88 (>0.10) | 78–96 | 94 (>0.10) | CD3 | 25–36 | 0 (>0.10) | 33–42 | 35 (<0.01) |
PMN | 0–5 | 2 (>0.10) | 1–5 | 3 (>0.10) | B220 | 1–10 | 5 (>0.10) | 1–16 | 8 (>0.10) | ||
EOS | 0–2 | 2 (>0.10) | 0–2 | 1 (>0.10) | DX5 | 0–8 | 5 (<0.01) | 3–12 | 7 (<0.01) | ||
LYM | 5–11 | 8 (>0.10) | 0–2 | 2 (>0.10) | RB6-8C5 | 2–10 | 5 (<0.05) | 1–9 | 6 (<0.01) | ||
CD11b | 8–15 | 10 (<0.01) | 2–10 | 6 (>0.10) | |||||||
pcDNA | CP52 | MAC | 80–95 | 87 | 70–94 | 92 | CD3 | 30–40 | 34 | 12–17 | 14 |
PMN | 1–5 | 3 | 0–12 | 3 | B220 | 0–4 | 2 | 4–10 | 6 | ||
EOS | 0 | 0 | 1–6 | 1 | DX5 | 30–38 | 35 | 37–54 | 48 | ||
LYM | 2–12 | 10 | 1–5 | 4 | RB6-8C5 | 8–28 | 22 | 19–25 | 22 | ||
CD11b | 25–40 | 38 | 3–10 | 6 |
Mice were i.n. infected with 104 PFU of virus or with pcDNA as described in Materials and Methods.
BAL was collected and analyzed by H&E staining for percent positive MAC, PMN, EOS, and LYM.
The nonparametric Mann-Whitney analysis was used to test for significant differences in percent cells between B1- and CP52-infected mice within each grouping. Total cell numbers in BAL of B1- and CP52-infected mice ranged from 2.0 × 105 to 3.5 × 105/ml and from 2.2 × 105 to 3.5 × 105/ml, respectively.
BAL was collected and analyzed by flow cytometry for percent CD3+ T cells, B220+ B cells, DX5+ NK cells, RB6-8C5 neutrophils, and CD11b+ PMN.