Fig. 3.

STAT3 is a central regulator of mTORC1 and CREB signaling. A, B Signature and heatmap of Stat3 and Stat3-regulated proteins in FFPE laser-microdissected prostates of 19-week-old WT, Pten^pc−/−, Pten^pc−/−Stat3^pc−/− and Pten^pc−/−Stat3^C/+ (n = 3 each) using unbiased LC–MS/MS proteomics. C The heatmap shows reprogramming of mTOR and CREB metabolic pathways in PCa with significant enrichment (hypergeometric test, q-value < 0.05). D Western blot analysis of p-CREB, CREB, p-4E-BP1, 4E-BP1, STAT3, p-S6, S6, AR and PSA of LNCaP xenograft tumors treated with vehicle or ruxolitinib (50 mg kg⁻¹) for 22 days. β-actin serves as a loading control. E Western blot analysis of p-CREB, CREB, AR, AR-V7 in 22Rv1 cells transfected with non-targeting (NT) or shRNAs specific for STAT3. Western blot analysis of STA3, p-CREB and CREB in PC3 cells transfected with an empty vector (EV) or STAT3 add-back. PC3 cells lacks STAT3 expression