TABLE 3.

Analysis of ex vivo CD8⁺ T-cell responses specific for autologous EBV-LCL eventually infected with vaccinia virus recombinants for various HIV-1_LAI genes

Patient	Medium		EBV		Vaccinia virus
	Frequencya	CD28b	Frequency	CD28	Wild type		Env		Gag		Pol		Nef
					Frequency	CD28	Frequency	CD28	Frequency	CD28	Frequency	CD28	Frequency	CD28
C03M	202	65	1,161	73	883	71	6,244	24	4,728	31	4,450	23	3,914	28
P04	258	22	2,142	20	1,663	24	7,674	12	3,296	16	2,555	18	1,282	21
Z050	87	69	1,572	71	868	59	1,141	41	1,970	29	2,270	26	2,866	37
Z077	224	15	3,494	60	1,749	52	7,726	15	7,492	21	18,000	10	5,060	27
Z100	188	26	5,976	50	2,810	42	6,018	18	20,225	16	9,825	22	2,551	38
Z108	333	62	1,392	74	760	62	1,147	39	7,867	17	2,960	27	923	48

^aNumber of CD8⁺ T cells secreting IFN-γ in response to antigen per 10⁶ total CD8⁺ lymphocytes. 

^bPercent CD28 expression on IFN-γ⁺ CD8⁺ T lymphocytes. Generally both a higher frequency of IFN-γ-secreting cells and a lower expression of CD28 attest to the specificity of the responses directed against EBV-LCL infected with vaccinia virus recombinants for HIV genes compared with those against EBV-LCL infected with wild-type vaccinia virus (values in boldface).