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. Author manuscript; available in PMC: 2023 Aug 12.
Published in final edited form as: Nature. 2023 Jul 19;619(7971):860–867. doi: 10.1038/s41586-023-06324-w

Extended Data Figure 2: Comparison of AT2 and iAT2 cells and their resulting tumors.

Extended Data Figure 2:

(a) Co-staining of early-stage AT1 adenomas shows absence of AT1 (LEL, AGER) and presence of AT2 (SFTPB, SFTPC) markers as well as cuboidal morphologies. (b) Feature plots localizing Axin2+ AT2 and iAT2 in single cell RNA sequencing data demonstrating co-clustering (above) with a heatmap of differentially expressed genes between the two groups (below). (c) Heatmap of the most differentially expressed genes between AT2>Kras cells 18d after Kras induction and early-stage AT1>Kras cells. Note on PAGA that the early-stage AT1 and AT2 tumor populations are distinct from normal AT2 and iAT2 (AT1>Kras) cells and that their trajectories diverge from each other.

Scale bars, 10 μm (a)

LEL, Lycopersicon esculentum lectin