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. 2023 Aug 12;13:13119. doi: 10.1038/s41598-023-40132-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Kaplan–Meier curves were generated to analyze overall survival (OS) (Fig. 5A,B), and disease-free survival (DFS) in the entire cohort of gastric cancer patients as well as stratified by sHLA-G expression levels (Fig. 5C,D). (A) The 12-month OS for the entire cohort was 49.9% with a median survival of 11.8 months. (B) Patients with high sHLA-G expression had a significantly lower 12-month OS compared to those with low expression. (C) The 12-month DFS for the entire cohort was 59.2% with a median survival of 15.9 months. (D) Patients with High HLA-G expression had a lower 12-month DFS those with low expression group (p-value > 0.05)., hierarchical cluster analysis using Ward's method of minimum variance was performed to determine that the cut-off point for sHLA-G levels was 39.95.