Fig. 3. GILG-WT specific T-cell repertoire analyses after peptide vaccination.

a Simpson’s diversity index of the WT-specific TCR repertoire after WT or CPL vaccination and influenza virus infection. b Skewing of the WT-specific TCR repertoire was calculated by the contribution of the top 5 largest clones per sample in percentages. c Overview of the number of shared sequences between mice vaccinated with WT or one of the four CPLs. TCR sequences were merged per group. d Overview of the number of GILG WT-specific TCR sequences shared between mice vaccinated with WT peptide or infected with influenza virus (PR8). e Overview of the percentage of sequences containing TRBV13–1. f Overview of the different JB segments withing the VB13+ sequences, TCR sequences were merged per group. a, b, e Results of individual mice are shown (points) with the group median (line). Mice vaccinated with WT peptide are depicted in black and mice vaccinated with CPL are indicated by the following colors: MOD1 – dark blue, MOD2 – green, MOD3 – orange, MOD4 – light blue. Mice infected with influenza a virus are shown as grey colored closed circles. Differences between responses to WT and CPLs are tested using Mann-Whitney U test. P-values are only shown when there was a significant difference between WT and a MOD vaccination.