Table 1.

Overview of peptide modifications and corresponding binding affinity to MHC.

	Amino Acid sequence + modification	Original name1	Binding2 (% of inhibition) after 4 h	Binding2 (% of inhibition) after 24 h
WT	GILGFVFTL		85 ± 0	84 ± 2
MOD1	[am-phg]ILGFVFTL	G1	97 ± 4	98 ± 4
MOD2	[3-PYRA]ILGFVFTL	G8	94 ± 3	93 ± 1
MOD3	G[NLE]LGFVFTL	G16	91 ± 3	90 ± 5
MOD4	[SOME]ILGFVFTL	G25	89 ± 12	89 ± 13

¹This data was previously published in the study of Rosendahl Huber et al. ⁷.

²HLA-A*0201 binding affinity was determined by a Fluorescence Polarization-Based Peptide Binding Assay⁷. Binding was scored as percentage inhibition of tracer peptide binding after 4 h and 24 h.