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. 2023 Aug 12;14(8):516. doi: 10.1038/s41419-023-06000-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A MTS assays measured the growth rates of UBC cells knocking down ANAPC11. ANOVA. B Representative images and histogram analysis of colony formation assays with T24 and UM-UC-3 cells treated with indicated siRNAs. Unpaired, two-tailed student’s t-test. C Representative images and quantification of cells in each phase within the cell cycle measured by flow cytometry. D The expression of ANAPC11 in UBC cells transfected with indicated plasmid measured by western blotting. E MTS assays of cells overexpressing ANAPC11. ANOVA. F Cell migratory and invasive abilities of UBC cells transfected with ANAPC11-NC or ANAPC11-OE plasmid were evaluated by Transwell migration and invasion assays. Unpaired, two-tailed student’s t-test. Scale bar = 100 μm. (G) Wound healing assays showed the migratory abilities of UBC cells. Unpaired, two-tailed student’s t-test. Scale bar = 200 μm. Data are shown as mean ± SD. ^**P < 0.01.