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. 2023 Aug 12;14(8):516. doi: 10.1038/s41419-023-06000-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Western blot analysis showed the abundance of FOXO3 in T24 and UM-UC-3 cells treated with indicated siRNAs. B Cell proliferation of UBC cells silencing FOXO3, shown by MTS assays. ANOVA. C Representative images and histogram analysis of colony formation assays with T24 and UM-UC-3 cells treated with indicated siRNAs. Unpaired, two-tailed student’s t-test. D Representative images and relative cell courts of Transwell migration and invasion assays. Unpaired, two-tailed student’s t-test. E Images of wound closure were captured, and the percentage of wound closure was demonstrated. Unpaired, two-tailed student’s t-test. Data are shown as mean ± SD. ^**P < 0.01.