Fig. 5. Overexpression of FOXO3 abrogates ANAPC11-mediated progression in UBC cells.

A–D ANAPC11-NC or -OE UBC cells were transiently transfected with pcDNA3.1 plasmids containing an NC sequence or the FOXO3 ORF. The growth rates of cells were evaluated by MTS assays (A); the colony formation abilities were measured by colony formation assays (B); the migration and invasion of cells were quantified by Transwell migration and invasion assays (C); and the percent of wound healing was evaluated via wound healing assays (D). Data are shown as mean ± SD. ANOVA, ^*P < 0.05, ^**P < 0.01. Scale bar = 100 μm (C). Scale bar = 200 μm (D).