| Antibodies |
|
| Camsap2 (rabbit polyclonal) |
Novus Biologicals |
Cat# NBP1-21402; RRID: AB_2068823 |
| γ Tubulin (rabbit polyclonal) |
Invitrogen |
Cat# PA1-28042; RRID: AB_2256789 |
| γ Tubulin (mouse monoclonal) |
Sigma-Aldrich |
Cat# T5326; RRID: AB_532292 |
| Tyr Tubulin (rat monoclonal) |
Millipore |
Cat# MAB1864; RRID: AB_2128189 |
| Acetylated Tub (Mouse monoclonal) |
Sigma-Aldrich |
Cat# T7451; RRID: AB_609894 |
| α-Tubulin (mouse monoclonal) |
Sigma-Aldrich |
Cat# T6199; RRID: AB_477583 |
| De-tyr Tubulin (Rabbit polyclonal) |
Millipore |
Cat# AB3201; RRID: AB_177350 |
| EB1 (Mouse monoclonal) |
BD Biosciences |
Cat# 610535; RRID: AB_397892 |
| Pericentrin (Rabbit polyclonal) |
Abcam |
Cat# Ab4448; RRID: AB_304461 |
| Centrin3 (Mouse monoclonal) |
Abnova |
Cat# H00001070-M01; RRID: AB_304461 |
| GM130 (Mouse monoclonal) |
BD Biosciences |
Cat# 610822; RRID: AB_398141 |
| IBA1 (Rabbit polyclonal) |
FujiFilm |
Cat# 019-19741; RRID: AB_839504 |
| Atto488 Phalloidin |
Sigma-Aldrich |
Cat# 49409 |
| Alexa Fluor 488 goat anti-mouse |
Invitrogen |
Cat# A11001; RRID: AB_2534069 |
| 488 goat anti-rat |
Invitrogen |
Cat# A11006; RRID: AB_141373 |
| 488 goat anti-rabbit |
Invitrogen |
Cat# A11008; RRID: AB_143165 |
| 594 goat anti-mouse |
Invitrogen |
Cat# A11032; RRID: AB_2534091 |
| 647 goat anti-rabbit |
Invitrogen |
Cat# A32733; RRID: AB_2633282 |
| CF 594 goat anti-rat |
Sigma-Aldrich |
Cat# SAB4600111 |
|
| Chemicals, peptides, and recombinant proteins |
|
| LPS from E.Coli O55:B5 |
Sigma-Aldrich |
Cat# L5418 |
| LPS from E.Coli O111:B4 |
Sigma-Aldrich |
Cat# L4391 |
| Murine recombinant IL-4 |
PEPROTECH |
Cat# 214-14 |
| Murine recombinant IFNγ |
PEPROTECH |
Cat# 315-05 |
| Taxol |
Sigma-Aldrich |
Cat# PHL89806
|
| Centrinone (PLK4 inhibitor) |
Tocris |
Cat# 5687 |
| Nocodazole |
Sigma-Aldrich |
Cat# 31430-18-9 |
| Hoechst |
Sigma-Aldrich |
Cat# 23491-45-4 |
| Propidium Iodide |
Sigma-Aldrich |
Cat# 25535-16-4 |
| Poly-L-lysine hydrobromide |
Sigma-Aldrich |
Cat# P6282 |
| Hexamethyldisilazane |
Sigma-Aldrich |
Cat# 999-97-3 |
| Dulbecco’s Modified Eagle’s Medium (DMEM) |
Sigma-Aldrich |
Cat# D6546 |
| Fetal bovine serum (FBS) |
Gibco |
Cat# 11573397 |
| Phosphate-Buffered Saline (PBS) tablets |
Sigma-Aldrich |
Cat# P4417 |
| Saponin |
Sigma-Aldrich |
Cat# 47036-50G-F |
| Lentiviral precipitation solution |
ALSTEM |
Cat# VC100 |
|
| Critical commercial assays |
|
| Quick RNA MiniPrep |
Zymo Research, Freiburg, DE |
Cat# R1055 |
| iScript Reverse Transcription Supermix |
Bio-Rad, Hercules, CA, USA |
Cat# 1708840 |
| Sybr Green |
Bio-Rad, Hercules, CA, USA |
Cat# 172-5124 |
| NuPAGE™ 4 to 12%, Bis-Tris, 1.0 mm, Mini Protein |
Invitrogen |
Cat# NP0321PK2 |
| SiR-Tubulin Kit |
Cytoskeleton |
Cat# CY-SC002 |
| Immun-Star Western Chemiluminescence Kit |
Bio-Rad |
Cat#170-5070 |
|
| Recombinant DNA |
|
| EB3-EGFP |
Pero et al.76
|
N/A |
|
| Deposited data |
|
| Max Intensity Radial Profile |
This paper |
https://github.com/ggosti/maxIntensityRadialProfile
|
|
| Experimental models: Organisms/strains |
|
| Mouse: C57BL/6 |
Charles River Laboratories |
027C57BL/6 |
| Mouse: Cx3cr1gfp/gfp
|
Jackson Laboratories |
B6.129P2(Cg)-Cx3cr1tm1Litt/J bred with C57BL6/j |
|
| Oligonucleotides |
|
| GAPDH forward: TCGTCCCGTAGACAAAAT GG |
Sigma-Aldrich |
Custom |
| GAPDH reverse: TTGAGGTCAATGAAGGGGTC |
Sigma-Aldrich |
Custom |
| Ym1 forward: CAGGTCTGGCAATTCTTCTGAA |
Sigma-Aldrich |
Custom |
| Ym1 reverse: GTCTTGCTCATGTGTGTAAGTGA |
Sigma-Aldrich |
Custom |
| Fizz1 forward: CCAATCCAGCTAACTATCCCTCC |
Sigma-Aldrich |
Custom |
| Fizz1 reverse: ACCCAGTAGCAGTCATCCCA |
Sigma-Aldrich |
Custom |
| Tnfα forward: GTGGAACTGGCAGAAGAG |
Sigma-Aldrich |
Custom |
| Tnfα reverse: CCATAGAACTGATGAGAGG |
Sigma-Aldrich |
Custom |
| IL1β forward: GCAACTGTTCCTGAACTCAACT |
Sigma-Aldrich |
Custom |
| IL1β reverse: ATCTTTTGGGGTCCGTCAACT |
Sigma-Aldrich |
Custom |
| iNOS forward: ACATCGACCCGTCCACAGTAT |
Sigma-Aldrich |
Custom |
| iNOS reverse: CAGAGGGGTAGGCTTGTCTC |
Sigma-Aldrich |
Custom |
|
| Software and algorithms |
|
| GraphPad Prism v9.0 |
GraphPad Software Inc. |
https://www.graphpad.com
|
| FIJI/ImageJ 1.53c |
Schindelin et al.77
|
http://www.imagej.nih.gov/ij
|
| ImageJ 1.50i |
Wayne Radband, National institute of Health, USA |
http://imagej.nih.gov/ij
|
| Metamorph 7.10.2 |
Molecular Devices |
https://www.moleculardevices.com/
|
| Imaris 8.1.2 |
Oxford Instruments |
https://imaris.oxinst.com/
|
| FlowJo v10.7.1 |
TreeStar, Ashland, OR, USA |
https://www.flowjo.com/
|
| Quantity One software |
Biorad |
https://www.bio-rad.com
|
| NIS-Elements AR 4.40.00 |
Nikon |
https://www.microscope.healthcare.nikon.com/
|
| Anaconda3 |
Anaconda Inc. |
https://www.anaconda.com
|
| Python 3.8.5 |
Python Software Foundation. |
http://www.python.org
|
| Numpy 1.19.2 |
Harris et al.78
|
https://numpy.org
|
| Matplotlib 3.3.2 |
Hunter,79
|
https://matplotlib.org
|
| Scikit-image 0.17.2 |
Van Der Walt et al.80
|
https://scikit-image.org
|
|
| Other |
|
| Killik O.C.T. Compound |
Bio-Optica |
Cat# 05-9801 |
| μ-Slide 8 Well |
Ibidi |
Cat# 80826 |
| Ibidi Mounting Medium |
Ibidi |
Cat# 50001 |
| ProLong Glass Antifade Mountant |
Invitrogen |
Cat# P36982
|
| Superfrost ultra plus slides |
Thermo Scientific |
Cat# 10149870 |
