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A continuous NS3-5A sequence is required for NS5A hyperphosphorylation. vTF7-3-infected BHK-21 cells were transfected with plasmids directing the expression of proteins given above the lanes, and HCV-specific proteins were analyzed after radiolabeling by immunoprecipitation under nondenaturing conditions, allowing the coprecipitation of NS4A with NS3. Specificities of antisera are given at the left; identities of the HCV proteins are shown at the right.
