FIG. 4.
Removal of two serine residues at the carboxy terminus of NS5AC does not affect hyperphosphorylation. (A) Amino acid sequence differences between NS5A of the C (shaded bar) and NC (light bar) genomes (see also Table 1). Arrows point to alanine residues substituting for serine residues of 5AC at positions 2368 and 2413. (B) Cells were transfected with constructs directing the expression of NS3-5BC polyproteins carrying the indicated amino acid substitutions within NS5AC. HCV-specific proteins were radiolabeled and analyzed by immunoprecipitation using antisera given to the right and SDS-PAGE. wt, wild type; DM, double mutant.