FIG. 6.

Hyperphosphorylation of NS5A is influenced by NS3. A schematic representation of the chimeric NS3-5B polyproteins expressed with the vaccinia virus-T7 hybrid system in BHK-21 cells is given above each lane. Thick lines refer to the C sequence; thin lines indicate the NC sequence. The individual constructs are identified above the lines. Schematic drawings of the corresponding C and NC polyproteins are given at the left and right, respectively, with dotted horizontal lines indicating the cleavage sites. Amino acid deviations of the NC sequence from the C sequence are marked with horizontal lines in the NC ORF. The phosphorylation patterns of NS5A proteins expressed from given constructs are shown below. The two phosphoprotein variants pp56 and pp58 are marked.