Comparison of isoprenylation of L as detected by 14C labeling in vivo and in vitro. Labeling was carried out in vivo, as for Fig. 1, or in vitro, using a coupled transcription-translation system. Samples were subject to electrophoresis as for Fig. 1, followed by electrotransfer and then direct quantitation of 14C with a bioimager. Lanes 1 and 3, the in vivo and in vitro samples, respectively; lane 2, a mixture of the two samples.