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. 2023 Aug 4;36:101751. doi: 10.1016/j.tranon.2023.101751

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© 2023 The Authors. Published by Elsevier Inc.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig 4 — BBOX1-AS1 bound to PFK1 mRNA and increased its stability. (A) BBOX1-AS1 was mainly expressed in the cytoplasm as determined by FISH assay. (B) BBOX1-AS1 was significantly positively correlated with PFKM expression in colon cancer as determined by GEPIA2 analysis. (C) BBOX1-AS1 was positively correlated with PFKM expression in the collected specimens. (D) PFK1 was significantly highly expressed in CRC as determined by RT-qPCR. (E) PFK1 was significantly highly expressed in CRC as determined by western blotting. (F) The expression of PFK1 in collected CRC samples as determined by RT-qPCR. (G) Immunohistochemistry confirmed the expression of PFK1 in colorectal cancer. (H) IntaRNA 2.0 predicted binding sites between BBOX1-AS1 and PFKM mRNA. (I) RIP assay verified that BBOX1-AS1 bound to PFK1 mRNA. (J) RT-qPCR showed that knocking down BBOX1-AS1 significantly downregulated PFK1 mRNA. (K) RNA stability experiments showed that BBOX1-AS1 affected PFK1 mRNA stability. *P < 0.05, **P < 0.01, ***P < 0.001.