FIG. 6.
vhs-induced RNA degradation is cap independent. (A) Unlabeled SRPα RNAs bearing the indicated 5′ cap structures were added to RRL-vhs (lanes vhs) and control RRL (lanes Retic), and samples were recovered at the indicated times (minutes). The RNA reaction products were then analyzed by primer extension with 5′ 32P-labeled oligonucleotide complementary to residues 60 to 84 of the SRPα RNA, as in Fig. 3. Solid arrowheads indicate the mobilities of the vhs-induced products. Numbers to the left of panel A indicate the sizes of DNA markers (lanes M) in nucleotides. (B) Internally labeled capped (7mGpppG) and uncapped SRPα RNAs were added to RRL containing vhs (lanes vhs) and RRL control (lanes Retic). Samples recovered at the indicated times (minutes) were then analyzed by agarose-formaldehyde gel electrophoresis. Numbers to the left of panel B indicate the sizes of RNA markers (lane M) in nucleotides.