Figure 1.

Single cell analysis of C134-treated mouse medulloblastomas

(A) Summary of orthotopic medulloblastoma mouse model experiments and survival data. Medulloblastoma tumors were established in the right cerebral cortices of immune-competent C57BL/6 mice. Five days after tumor implantation, the mice received 1 × 10⁷ pfu of C134 or vehicle. Mice were then either followed for survival studies, or on days 2 or 6 post-treatment, sacrificed, and brain tumors were harvested and processed for downstream sample characterization experiments. Kaplan-Meier curves depict differences in survival and statistical differences determined using the log rank test. UMAP plots show annotated cell types from single cell RNA sequencing of mouse medulloblastomas for the overall dataset (B), immune cell subset (D), and macrophage subset (F). Accompanying dot plots show normalized expression of marker genes by cell types in the overall dataset (C), the immune cell subset (E), and macrophage subset (G).